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Quick Links:   IVIS Blog | Imaging Tech-Notes | Living Image 3.x Tech-Notes | Living Image 4.x Tech-Notes | Protocols

 IVIS Blog

   Click here to access the IVIS Blog site  
   

IVIS iTunes App (iPhone, iPod, iPad)

 

          iTunes App Specs

 

          Download FREE iTunes App

 
   

 Imaging Tech-Notes

   

Acquisition of High Resolution Images

   

Determine Areas of Saturation

   

Filter recommendations for common fluorescent proteins, dyes and Quantum Dots using the IVIS Lumina and IVIS Kinetic equipped with the standard filter set

   

Filter recommendations for common fluorescent proteins, dyes and Quantum Dots using the IVIS Spectrum and Lumina II or IVIS Kinetic equipped with spectral unmixing filters

   

 IVIS Camera Cleaning and Sterilization Information

     

 Living Image® 3.X Tech-Notes

    Diffuse Light Imaging Tomography (DLIT)
    DLIT Sequence Acquisition
    Drawing ROIs
    FLIT Sequence Acquisition
    Fluorescence Tomography
    Imaging Wizard
    Loading and Analyzing Groups of Images
    Spectral Unmixing
    Surface Topography
    Transillumination Sequence Setup
    Using 96 Well Plates with Transillumination
    Optical 3D-Imaging Co-Registration with MRI and CT in Living Image 3.1
    Acquire Image with an IVIS
    Data Analysis using Region of Interest (ROI)
     

 Living Image® 4.x Tech-Notes

 

Adaptive Fluorescence Background Subtraction

 

Auto-exposure automatically sets the exposure time, f/stop and binning to keep the signal within an optimal range for quantification

 

DLIT Sequence Acquisition

 

Diffuse Light Imaging Tomography (DLIT)

 

Drawing ROIs

 

Drawing and Using Subject ROIs

 

FLIT Sequence Acquisition

 

Identify Saturated Pixels in an Image

 

Image Overlay – 2D

 

Image Overlay - 3D

 

Imaging Wizard

 

Loading and Analyzing Groups of Images

 

Normalized Transmission Fluorescence

 

Planar Spectral Imaging Sequence Acquisition

 

Spectral Unmixing Manual Setup

 

Subtracting Background ROI from a Sequence of Images

 

Surface Topography

 

Transillumination Sequence Setup Quick Reference Guide

 

Transillumination Raster Scan

 

Transillumination of 96 Well Plates

 

Well Plate Quantification

     

 Protocols

 

Proper Method for Intraperitoneal Injections

 

Stable Transfection of Adherent Cells

 

Determining the Luciferin Kinetic Cruve for Your Model

 

Preparation of Luciferin for In Vivo Bioluminescent Assays

 

Intraperitoneal (i.p.) Injection of Luciferin

 

Intravenous (i.v.) injection of Luciferin

 

Cell Preparation for Injection to Animals

 

Injection of Tumor Cells into Animals

 

Subcutaneous injection of tumor cells into animals

 

Intravenous injection of tumor cells into C57Bl/6 Albino mice

 

Small Animal Anesthesia Protocol