Bioware® Light Producing Cells

Oncology
Caliper applications in oncology through light producing cell lines used in conjunction with IVIS® Imaging Systems offer unique opportunities to measure tumor growth and metastasis in a variety of models.  In vivo bioluminescent imaging allows the non-invasive detection and quantification of orthotopic, metastatic, and spontaneous tumors in the whole mouse.  Extensive in house studies indicate that light emission from tumors growing in rodents is directly proportional to tumor mass measured by mechanical means (caliper and weight) over the wide range of tumor sizes.  Caliper's biophotonic imaging technology has been optimized for high sensitivity, such that micrometastases can be detected that would otherwise require histopathology to identify.  Caliper oncology models can be used to assess anti-cancer therapies over the course of treatment in vivo.

• - Quantify tumor burden in the whole mouse
• - Detect micrometastases non-invasively in living animals with high sensitivity
• - Visualize metastases spontaneously generated from a primary tumor
• - Follow responses to therapeutic treatments non-invasively in longitudinal studies

Breast, Lung, and Colon Carcinoma Models
Caliper light producing cell line models can be used to establish orthotopic mammary gland tumors, experimental lung metastasis by intravenous injection or spontaneous metastasis from a primary tumor site, or establish subcutaneous or metastatic tumor models.

Signal Transduction/Mechanism-Based Reporters
Caliper light producing cell line models are also available that link luciferase expression to signal transduction pathways that are key to the oncogenic process. These models offer the ability to track activation of factors that signal cell death or survival, or angiogenesis.